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Strep Tag II Mouse Monoclonal Antibody
貨號:PMK562M/PMK562S
一鍵復制產品信息
價格:
580.00
規格:
50ul
100ul
貨期:
現貨
本產品4℃運輸,保存于-20℃,保質期 12個月。
Details
產品詳情
  • Catalog:

    PMK562M               PMK562S

  • Quantity:

      50ul                       100ul

  • Applications:

    WB, IHC

  • Species Cross-Reactivity:

    Recombinant Strep Tag II Specific

  • Molecular Weight:

    Strep Tag II

  • Isotype:

    IgG1

  • Storage Buffer & Condition:

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions:

    WB: 1:5,000   IHC: 1:200 

  • Specificity:

    The Strep Tag II Mouse Monoclonal Antibody can detects Strep Tag II recombinant protein.

  • Background:

    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological 

    protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity 

    and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag 

    II fusion proteins.


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  • Application:

    WB-Western blot

    IHC-Immunochemistry

    IF-Immunofluorescence

    IP-Immunoprecipitation

    ChIP-Chormatin Immunoprecipitation


  • Reactivity:

    H-Human      R-Rat               M-Mouse       Mk-Monkey    Dg-Dog

    Ch-Chicken   Hm-Hamster   Rb-Rabbit       Sh-Sheep        Pg-Pig


Instruction manual/COA download
說明書/COA下載
Published literature
已發表文獻
  • 更新中...

Product Q&A FAQ
產品問答FAQ
  • Western Blot(WB)實驗中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個修飾位點,本身可以呈現多條帶,建議查閱文獻或進行生物信息學分析,獲得蛋白序列的修飾位點信息,通過去修飾確定蛋白實際大小;

    2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑;樣本處理時在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強,建議使用特異性強的抗體;

    5、抗體孵育時間過久,建議減少抗體孵育時間;

    6、二抗與抗原有交叉反應,建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質變性過程及強度;

    8、底物顯色與曝光時間過長,建議縮短顯色及曝光的時間。


Copyright ?湖北普美生物科技有限公司 All Rights Reserved.    工信部備案:鄂ICP備17001029號-1    技術支持:武漢微盟

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