WB, IHC
Mouse
N/A
IgG1
PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
1:500-1:5,000 for immunohisto/cytochemistry
1:5,000-1:100,000 for ELLSA and Western blotting with chromogenic substrates
1:10,000-1:200,000 for Western blotting with ECL substates
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also reacts with the
light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.The
antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the
intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
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9. Peipei Y ,Lanlan D ,Qingqing C , et al.Prototype foamy virus elicits complete autophagy involving the ER stress-related UPR pathway.[J].Retrovirology,2017,14(1):16. (IF 3.867)
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12. Tan WL ,Yu X ,Jia J , et al.Alpha-lipoamide prevents acute kidney injury in mouse by inhibiting renal tubular epithelial cell pyroptosis.Biochem Pharmacol.2025;237:116942. (IF 5.6)
13. Zhang, Fan, et al.Activation of IRF2 signaling networks facilitates podocyte pyroptosis in lupus nephritis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2025): 167990. (IF 4.2)
14. Jianwei Xu, Zhangxiang Yang, Fuhui Zhao, Design, synthesis, and biological evaluation of novel GSK-3β covalent inhibitors for cancer treatment. (IF 4.7)
答：可能的原因有：
1、目的蛋白有多個(gè)修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過(guò)去修飾確定蛋白實(shí)際大小；
2、樣本處理過(guò)程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過(guò)久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過(guò)程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過(guò)長(zhǎng)，建議縮短顯色及曝光的時(shí)間。
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