WB,IF,ELISA,IHC,Fc
Rabbit
N/A
IgG1
PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:10000-1:50000
IHC 1:500 - 1:5000
IF:1:200-1:1000
The antibody was purified from antisera by immunoaffinity chromatography using antigens
coupled to agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule Rabbit IgG. lt also
reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Jun-qin Z ,Yan-ming W ,Kun H , et al.Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome[J].Journal of Integrative Agriculture,2019,18(11):2598-2604.(IF 1.337)
2.Fang X Y ,Lu Q E ,Xu E , et al.Arf1 promotes porcine intestinal epithelial cell proliferation via the mTORC1 signaling pathway[J].In Vitro Cellular & Developmental Biology - Animal,2024,60(9):1-12.(IF 1.5)
3.Jiang J ,Yang P ,Xu X , et al.Donafenib inhibits PARP1 expression and induces DNA damage, in combination with PARP1 inhibitors promotes apoptosis in liver cancer cells.[J].Anti-cancer drugs,2024,(IF 1.8)
4.Zhu M ,Fang Y ,Cheng Y , et al.The Alleviating Effect of Taxifolin on Deoxynivalenol-Induced Damage in Porcine Intestinal Epithelial Cells.[J].Veterinary sciences,2024,11(4):156-.(IF 2)
5.Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
6.Xin W ,MingJing H ,XiaoJie C , et al.Glaucocalyxin A impairs tumor growth via amplification of the ATF4/CHOP/CHAC1 cascade in human oral squamous cell carcinoma.[J].Journal of ethnopharmacology,2022,290115100-115100.(IF 5.195)
7.Ling Y ,YongXia F ,EnQing L , et al.Extracellular Glutamine Promotes Intestinal Porcine Epithelial Cell Proliferation via Arf1-mTORC1 Pathway Independently of Rag GTPases.[J].Journal of agricultural and food chemistry,2023,71(39):(IF 6.1)
答：可能的原因有：
1、目的蛋白有多個(gè)修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過去修飾確定蛋白實(shí)際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過長(zhǎng)，建議縮短顯色及曝光的時(shí)間。
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