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C-Myc-Tag Rabbit Polyclonal Antibody
貨號(hào):PMK112M/PMK112S
一鍵復(fù)制產(chǎn)品信息
價(jià)格:
580.00
規(guī)格:
50ul
100ul
貨期:
現(xiàn)貨
本產(chǎn)品4℃運(yùn)輸,保存于4℃,保質(zhì)期 12個(gè)月。
Details
產(chǎn)品詳情
  • Applications

    WB

  • Species Cross-Reactivity

    N/A

  • Molecular Weight

    N/A

  • Isotype

    IgG

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions

    WB: 1:5,000  

  • Specificity

    The C-Myc tag antibody can detect C-Myc fusion proteins.

  • Background

    c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a 

    transcription factor that activates expression of a great number of genes through binding on consensus 

    sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent 

    tudy demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a 

    potential cancer drug target.


Instruction manual/COA download
說(shuō)明書/COA下載
Published literature
已發(fā)表文獻(xiàn)
  • 更新中...

Product Q&A FAQ
產(chǎn)品問(wèn)答FAQ
  • Western Blot(WB)實(shí)驗(yàn)中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個(gè)修飾位點(diǎn),本身可以呈現(xiàn)多條帶,建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析,獲得蛋白序列的修飾位點(diǎn)信息,通過(guò)去修飾確定蛋白實(shí)際大小;

    2、樣本處理過(guò)程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑;樣本處理時(shí)在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強(qiáng),建議使用特異性強(qiáng)的抗體;

    5、抗體孵育時(shí)間過(guò)久,建議減少抗體孵育時(shí)間;

    6、二抗與抗原有交叉反應(yīng),建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質(zhì)變性過(guò)程及強(qiáng)度;

    8、底物顯色與曝光時(shí)間過(guò)長(zhǎng),建議縮短顯色及曝光的時(shí)間。


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