
WB

N/A

N/A

IgG

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.

WB: 1:5,000

The C-Myc tag antibody can detect C-Myc fusion proteins.

c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a
transcription factor that activates expression of a great number of genes through binding on consensus
sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent
tudy demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a
potential cancer drug target.
更新中...
答:可能的原因有:
1、目的蛋白有多個(gè)修飾位點(diǎn),本身可以呈現(xiàn)多條帶,建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析,獲得蛋白序列的修飾位點(diǎn)信息,通過(guò)去修飾確定蛋白實(shí)際大小;
2、樣本處理過(guò)程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑;樣本處理時(shí)在冰上操作;
3、雜蛋白多,建議處理目的蛋白;
4、抗體特異性不強(qiáng),建議使用特異性強(qiáng)的抗體;
5、抗體孵育時(shí)間過(guò)久,建議減少抗體孵育時(shí)間;
6、二抗與抗原有交叉反應(yīng),建議選擇合適的封閉物;
7、二聚體或多聚體存在,建議增加蛋白質(zhì)變性過(guò)程及強(qiáng)度;
8、底物顯色與曝光時(shí)間過(guò)長(zhǎng),建議縮短顯色及曝光的時(shí)間。
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