WB, IHC, IF
H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
37KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IF: 1:100-1:500
Antibody can detects endogenous GAPDH protein.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis.
GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as
loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH
expression in certain cell types.
1.Xiao-Long W ,Shuo W ,Zhi-Zhong W , et al.Overexpression of ATAD2 indicates Poor Prognosis in Oral Squamous Cell Carcinoma.[J].International journal of medical sciences,2020,17(11):1598-1609.(IF 2.523)
2.Bing H ,Yi Y ,Lei T , et al.Roles of SET7/9 and LSD1 in the Pathogenesis of Arsenic-induced Hepatocyte Apoptosis.[J].Journal of clinical and translational hepatology,2021,9(3):364-372.(IF 4.108)
3.Liyuan H ,Fangfang S ,Hualing S , et al.IRX5 promotes NF-κB signalling to increase proliferation, migration and invasion via OPN in tongue squamous cell carcinoma.[J].Journal of cellular and molecular medicine,2018,22(8):3899-3910.(IF 4.302)
4.Xueqing Z ,Wushuang H ,Zhenru H , et al.Effects of Fam83h truncation mutation on enamel developmental defects in male C57/BL6J mice.[J].Bone,2022,166116595-116595.(IF 4.626)
5.De-Run C ,Yuan G ,Yao X , et al.Engineered nanogels simultaneously implement HDAC inhibition and chemotherapy to boost antitumor immunity via pyroptosis[J].Applied Materials Today,2022,26(IF 8.663)
6.Chengcan Y ,Qian G ,Nuo X , et al.Human dental pulp stem cells are subjected to metabolic reprogramming and repressed proliferation and migration by the sympathetic nervous system via ADRA1B.[J].Journal of endodontics,2023,49(12):1641-1651.e6.(IF 4.2)
7.Wang S ,Wang S ,Sun X , et al.AGO2 Negatively Regulates Type I Interferon Signaling Pathway by Competition Binding IRF3 with CBP/p300[J].Frontiers in Cellular and Infection Microbiology,2017,7195. (IF 4.3)
8.Shengyu W ,Zhenrong L ,Yaping C , et al.ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62.[J].Virology journal,2023,20(1):58-58. (IF 4.8)
9.Wushuang H ,Xueqing Z ,Mei Y , et al.PER2-mediated ameloblast differentiation via PPARγ/AKT1/β-catenin axis.[J].International journal of oral science,2021,13(1):16-16. (IF 6.344)
10.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.575)
11.Fei W ,Chong Y ,Bo L , et al.Syringin exerts anti-breast cancer effects through PI3K-AKT and EGFR-RAS-RAF pathways[J].Journal of Translational Medicine,2022,20(1):310-310. (IF 8.44)
12. Jinmeng Sun, Minmin Sun, Zekun Li, GelMA@ginsenoside Rb3 Targets Inflammatory Microenvironment in Periodontitis via MAPK Pathway. (IF 5.3)
答：可能的原因有：
1、目的蛋白有多個修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過去修飾確定蛋白實(shí)際大小；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時(shí)在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時(shí)間過久，建議減少抗體孵育時(shí)間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強(qiáng)度；
8、底物顯色與曝光時(shí)間過長，建議縮短顯色及曝光的時(shí)間。
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