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Goat anti-Rat IgG (H+L), HRP Conjugated
貨號:PMK-014-099M/PMK-014-099S
一鍵復制產品信息
價格:
160.00
規格:
100ul
1ml
貨期:
現貨
本產品4℃運輸,保存于-20℃,保質期 12個月。
Details
產品詳情
  • Applications

    WB, IHC

  • Species Cross-Reactivity

    Rat

  • Molecular Weight

    N/A

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and  50% Glycerol. Store at -20°C. 

    Do not aliquot the antibody.


  • Suggested Dilution Range

    1:500-1:5,000 for immunohisto/cytochemistry

    1:3,000-1:100,00 for ELLSA and Western blotting with chromogenic substrates 

    1:50,000-1:100,00 for Western blotting with ECL substates


  • Purity

    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Antibody Specificity:Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule rat IgG. 

    lt also reacts with the light chains of other rat immunoglobulins. No antibody was detected against 

    non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

    Expiration date:Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results 

    are acceptable for the intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device. 

Instruction manual/COA download
說明書/COA下載
Published literature
已發表文獻
  • 更新中...

Product Q&A FAQ
產品問答FAQ
  • Western Blot(WB)實驗中有很多雜帶咋回事?

    答:可能的原因有:

    1、目的蛋白有多個修飾位點,本身可以呈現多條帶,建議查閱文獻或進行生物信息學分析,獲得蛋白序列的修飾位點信息,通過去修飾確定蛋白實際大小;

    2、樣本處理過程中目的蛋白發生降解,建議加入蛋白酶抑制劑;樣本處理時在冰上操作;

    3、雜蛋白多,建議處理目的蛋白;

    4、抗體特異性不強,建議使用特異性強的抗體;

    5、抗體孵育時間過久,建議減少抗體孵育時間;

    6、二抗與抗原有交叉反應,建議選擇合適的封閉物;

    7、二聚體或多聚體存在,建議增加蛋白質變性過程及強度;

    8、底物顯色與曝光時間過長,建議縮短顯色及曝光的時間。


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