Flow Cyt,ICC/IF
Chicken
150KD
IgG
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6.Store at -20°C.
Do not aliquot the antibody.
2mg/ml
Goat Polyclonal IgG, affinity purified by Protein A.
Flow-Cyt=1:100-1000 IF=1:100-1000
Fluorescein-5-isothiocyanate(FITC)Amax=495nm;Emax=519nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult
blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a
part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited
the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by
somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for
the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Zhou Y ,Dong W ,Wang L , et al.Cystatin C Attenuates Perihematomal Secondary Brain Injury by Inhibiting the Cathepsin B/NLRP3 Signaling Pathway in a Rat Model of Intracerebral Hemorrhage.[J].Molecular neurobiology,2024,61(11):9646-9662. (IF 4.6)
2.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.757)
3.Zhao Y ,Wang P ,Sun X , et al.Candida albicansInfection Disrupts the Metabolism of Vaginal Epithelial Cells and Inhibits Cellular Glycolysis[J].Microorganisms,2024,12(2): (IF 4.1)
4.Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
答：可能的原因有：
1、目的蛋白有多個修飾位點(diǎn)，本身可以呈現(xiàn)多條帶，建議查閱文獻(xiàn)或進(jìn)行生物信息學(xué)分析，獲得蛋白序列的修飾位點(diǎn)信息，通過去修飾確定蛋白實(shí)際大??；
2、樣本處理過程中目的蛋白發(fā)生降解,建議加入蛋白酶抑制劑；樣本處理時在冰上操作；
3、雜蛋白多，建議處理目的蛋白；
4、抗體特異性不強(qiáng)，建議使用特異性強(qiáng)的抗體；
5、抗體孵育時間過久，建議減少抗體孵育時間；
6、二抗與抗原有交叉反應(yīng)，建議選擇合適的封閉物；
7、二聚體或多聚體存在，建議增加蛋白質(zhì)變性過程及強(qiáng)度；
8、底物顯色與曝光時間過長，建議縮短顯色及曝光的時間。
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